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CanadianStandardReagents.com
CSR Hot-Start TAQ DNA Polymerase, with 10x Reaction Buffer (no dNTP)
CSR Hot-Start TAQ DNA Polymerase, with 10x Reaction Buffer (no dNTP)
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$330.00
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$330.00
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Description
For Research Use Only. Not for use in diagnostic procedures.
The CSR HotStart TAQ DNA Polymerase reliably amplifies a wide variety of DNA templates—including mouse, human, plasmid, and bisulfite-converted DNA—producing consistent PCR products. This polymerase leaves a 5′ adenine overhang, making the products well-suited for TA cloning. Verified extension capabilities reach up to 5,000 base pairs, with speeds of up to 1 kb per minute.
Hot-Start Technology
Hot-Start TAQ DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3’ cap that prevents its amplification and ensures no interference with cloning or any downstream analysis.
Specifications
- Generates PCR product with 3'-dA overhangs
- The error rate in PCR is 2.2 x 10-5 errors per nucleotide per cycle
- Generates PCR product of up to 5 kb
- Aptamer based Hot-Start provides a reversible inhibition of non-specific PCR products
Applications
- Effective in routine and high throughput PCR
- Incorporates modified nucleotides in PCR.
- Effective in DNA labeling.
Kit Components
- 10X PCR Buffer
- 50 mM MgSO4
- GC Enhancer (For GC-rich templates and difficult templates)
- DNase/ RNase/ Protease free water.
- Protocol
Documentation
- SDS Sheets*
- Certificate of Authenticity (COA)*
*Contact us for SDS sheets or COA
For Research Use Only. Not for use in diagnostic procedures.
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